DNA

Part:BBa_K4245131:Design

Designed by: Fulin Zhou, Baoxin Li, and Jiyuan Maa   Group: iGEM22_Lambert_GA   (2022-09-23)

Design Notes

The middle sequence was designed by researchers at Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province at Shaanxi Normal University. For the purposes of Lambert iGEM, the middle sequence was incorporated into the padlock probe designs for hsa-miR-1-3p RCA Padlock Probe (BBa_K4245200), hsa-miR-122-5p RCA Padlock Probe (BBa_K4245202), hsa-miR-126-3p RCA Padlock Probe (BBa_K4245203), hsa-mir-133a-3p RCA Padlock Probe (BBa_K4245204), hsa-miR-150-5p RCA Padlock Probe (BBa_K4245206), hsa-miR-208a-3p RCA Padlock Probe (BBa_K4245207), and hsa-mir-451a RCA Padlock Probe (BBa_K4245209).


Source

The middle sequence was incorporated into the padlock probe designs for RCA, and the sequence was ordered as a DNA oligo from Integrated DNA Technologies.


Middle sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]